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NH4+ turnover in intertidal sediments of Marennes-Oleron Bay (France): effect of sediment temperature ArchiMer
Vouve, Florence; Guiraud, Gérard; Marol, Christine; Girard, Marine; Richard, Pierre; Laima, Mario.
NH4+ turnover plays an important role in benthic compartment as source of nutrient for primary production. The intertidal mudflats of the Marennes-Oleron Bay are characterized by high fluctuations of sediment temperature that generally ranges from 10 to 30 degreesC during emersion periods. Influence of sediment temperature on NH4+ processes was investigated. Grass ammonification and NH4+ incorporation kinetics were studied using the N-15 isotope dilution method. The method was first adapted to these very fine sediments and applied to 2 upper sediment layers (0-2 and 2-4 cm) at different incubation times of up to 120 h. The effect of sediment temperature on gross ammonification and NH4+ incorporation rates was investigated at 10, 20 and 30 degreesC. There...
Tipo: Text Palavras-chave: N 15; Intertidal sediments; Sediment temperature; NH4+ turnover; 15N; Sédiments intertidaux; Température du sédiment; Régénération du NH4 +.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/2000/publication-530.pdf
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Optimising extraction of extracellular polymeric substances (EPS) from benthic diatoms: comparison of the efficiency of six EPS extraction methods ArchiMer
Takahashi, Eri; Ledauphin, Jerome; Goux, Didier; Orvain, Francis.
There is no universal method that can be applied to extract bound extracellular polymeric substances (EPS) from benthic diatoms of intertidal sediments without causing cell lysis. Six extraction methods were tested on a diatom culture of Navicula jeffreyi to establish the best compromise between high yields of carbohydrate extraction and minimum cell lysis. Extraction with distilled water provoked cell lysis (as already known). The five other extraction methods (dowex resin, artificial seawater of half salinity and extractions after pretreatment with gluteraldehyde by three methods: water, dowex water and dowex buffer) did not provoke cell lysis as shown by transmission electronic microscopy. This result was confirmed by the minimum release of internal...
Tipo: Text Palavras-chave: Transmission electron microscopy; Protein; Navicula jeffreyi; Intertidal sediments; Immuno staining; Immunostaining; GCMS analysis; Dowex; Cell lysis; Biofilm.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-7418.pdf
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Temporal variability in the biochemical composition of sedimentary organic matter in an intertidal flat of the Galician coast (NW Spain) ArchiMer
Cividanes, S; Incera, M; Lopez, J.
Temporal variations and spatial distribution of sedimentary organic matter composition were investigated over a one year period in an intertidal flat of the NW Spain. Sediment samples were collected from 0 to 25 cm depth, every three months, from January 1997 to January 1998 at three tidal levels (high, medium and low). Changes in the elemental and biochemical composition were assessed to gather information on temporal and spatial fluctuations in quantity and quality of sedimentary organic matter potentially available to benthic deposit-feeder nutrition. Organic matter content was significantly higher at the medium tidal level, while minimum values were found at the high tidal level. The different biochemical classes of organic compounds exhibited...
Tipo: Text Palavras-chave: Matière organique sédimentaire; Lipide; Protéine; Hydrate de carbone; Sédiment intertidal; Sedimentary organic matter; Lipids; Proteins; Carbohydrates; Intertidal sediments.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00322/43324/42930.pdf
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The effect of tidal range on the flushing of ammonium from intertidal sediments of the Tagus estuary, Portugal ArchiMer
Cabrita, Mt; Catarino, F; Vale, C.
The time-course evolution of ammonium concentration has been examined in the flood water during the first 25 min of tidal inundation. The way this transport fluctuates with the tidal ranges and wind conditions was investigated. Flood water was collected at three sites, located along a transect from the lower to the upper intertidal area of the Tagus estuary. At spring and intermediate tides, the periods of air exposure vary slightly along the transect due to the high tidal amplitude and the flatness of the area, but the upper site remains uncovered at neap tide over the entire tidal cycle. At each site, sampling was performed at different tidal ranges covering the neap-spring tidal cycle and wind conditions. Ammonium was determined in the flood water at...
Tipo: Text Palavras-chave: Extraction par la marée; Ammonium; Sédiment intertidal; Estuaire; Tidal flushing; Ammonium; Intertidal sediments; Estuary.
Ano: 1999 URL: http://archimer.ifremer.fr/doc/00325/43611/43908.pdf
Registros recuperados: 4
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